Plant Pathology Journal

Volume 21 (1), 24-32, 2022


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Potential of Consortium of Native Microbiota Against Damping-off of Cedrus deodara Seedlings in North-Western Himalayan Region of India

Manica Tomar, Abhishek Chauhan, Manisha Thakur and Savita Jandaik

Background and Objective: Soil-borne pathogens causing damping-off in the nursery can be managed by isolating and evaluating native biocontrol agents from the soil. The present study aimed at the isolation and preparation of a consortium of biocontrol agents against the damping-off pathogen. Materials and Methods: The native biocontrol agents were isolated from the soil, identified and purified. These native biocontrol agents were used against the damping-off pathogen in vitro and the four best biocontrol agents were selected for making a potent consortium against Fusarium oxysporum f. sp. pini. Results: Trichoderma virens, Trichoderma viride, Trichoderma harzianum, Trichoderma hamatum, Aspergillus sp., Penicillium sp., Bacillus subtilis and Pseudomonas fluorescens were some of the microflora isolated and purified for testing against the pathogen. In vitro evaluation of the isolated biocontrol agents against the pathogen revealed that Trichoderma virens, Trichoderma harzianum, Trichoderma viride and Penicillium sp. were effective against the damping-off the pathogen. The consortium of four best fungal strains tested in vitro was prepared and inoculated in the nursery soil to test its efficacy in vivo against the damping-off of Cedrus deodara. Trichoderma harzianum +Penicillium sp.+Trichoderma virens showed maximum disease incidence reduction with only 29.44% incidence followed by integration of Trichoderma viride +Trichoderma virens +Penicillium sp. (31.33%) and Trichoderma viride +Trichoderma harzianum +Penicillium sp. (33.85%), respectively. Conclusion: The results highlight the potential of bio control agents present in the soil against the soil-borne pathogens causing severe diseases and hampering the production of healthy forests.

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How to cite this article:

Manica Tomar, Abhishek Chauhan, Manisha Thakur and Savita Jandaik, 2022.

Potential of Consortium of Native Microbiota Against Damping-off of Cedrus deodara Seedlings in North-Western Himalayan Region of India

. Plant Pathology Journal, 21: 24-32.
DOI: 10.3923/ppj.2022.24.32
URL: https://ansinet.com/abstract.php?doi=ppj.2022.24.32

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