Comparison of Two Egyptian Isolates of Spiroplasma citri by Crossed Immunoelectrophoresis and Polyacrylamide Gel Electrophoresis of Cell Proteins

Ayman Omar, Kamaruzman Sijam, Inon Sulaiman, Habibuddin Hashim and Om-hashem Banna

Differences between two Spiroplasma citri isolates were detected by crossed immunoelectrophoresis (CIE) with intermediate gel and polyacrylamide gel electrophoresis. In the homologous reactions of Fewa isolate, eleven precipitin peaks were detected using CIE. Identical homologous reaction of Qualubia isolate was produced. One antigen (a) was specific for the Fewa isolate and one antigen (b) was specific for the Qualubia isolate when CIE with intermediate gel was used. One-dimensional electrophoresis analysis demonstrates very similar patterns of protein with two different protein bands between the two isolates of S. citri.