Development of Experimental in vitro Models to Elucidate Molecular Mechanisms Underlying Pathology and its Prevention

Iskra Sainova, Vera Kolyovska, Elica Mihaylova, Desislava Drenska, Dimitar Maslarov, Dimitrina Dimitrova-Dikanarova, Radka Hadjiolova and Tzvetanka Markova

Background and Objective: Pathological processes at the cellular level can cause damage and understanding their underlining molecular mechanisms is crucial. The study aims to develop experimental in vitro models to investigate these mechanisms and identify potential strategies for preventing pathology. Materials and Methods: The average titers of gangliosides and anti-ganglioside antibodies in samples of non-malignant mouse embryonic cells 3T3 fibroblasts, of mouse malignant myeloma cells and mixed of the two cell types in vitro-cultures were assessed as homogenates of each one of the three types. The tested in vitro-cultures were applied in the role of experimental in vitro-models of patients with multifactor diseases in different phases, as well as of a healthy human organism. Results: The assessed varieties in the average titers of the gangliosides and anti-ganglioside antibodies could be due to participation of these molecules in different in various intra- and extra-cellular inter-molecular interactions. Production of antibodies/immunoglobulins by non-lymphoid and non-hematopoietic types of cells in appropriate conditions, but also about differentiation of non-immune and non-hematopoietic cell progenitors to myeloid and lymphoid directions in appropriate conditions, were proposed. Conclusion: Activation of internal protective mechanisms was suggested. Further studies should be performed.

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