Laccase Activity of Trametes versicolor Using Various Pineapple and Arabica Coffee Wastes Under Solid-State Fermentation Process

Tri Yuliana, Salsabila Zaharani, Indira Lanti, Herlina Marta, Ratu Safitri and Annisa Krama

Background and Objective: Laccase, a ligninolytic enzyme, can degrade lignin by utilizing lignocellulose. With this ability, laccase is useful as a pre-treatment enzyme of lignocellulosic materials in various industries. This study evaluated the laccase activity produced by T. versicolor using pineapple and coffee-derived waste as lignocellulosic-rich substrates using solid fermentation. Materials and Methods: This study tested laccase production activities from various pineapple wastes and coffee peels through a solid fermentation process by growing T. versicolor on the substrate mixture of Szabo media and pineapple skin or coffee peel. The laccase produced was analyzed for its activity and growth, qualitatively and quantitatively during a 12 days incubation time. The method used was experimental followed by descriptive analysis. Results: Various amounts of lignocellulose were obtained among pineapple wastes and coffee peel. Laccase from these substrates was optimally produced on day 6 incubation on Szabo+5 g coffee peel group showed the highest laccase activity levels, following its biomass weight (1949.13 U/L and 3.498 g). The optimum pH for laccase production was reached in the range 4-6. The produced laccase was indicated by the appearance of the blackish-brown zone on PDA agar supplemented with guaiacol. Conclusion: Lignocellulosic wastes from pineapple and coffee had the potential as substrates to produce laccase from T. versicolor with various activity levels which day 6 incubation showed the most optimum fermentation period.

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