Antioxidant and Anticoagulant Activity of Microbial Nano Cellulose-ZnO-Ag Composite Components

Amr A. El-Waseif, Fatimah Alshehrei, Saleh B. Al-Ghamdi and Dina E. El-Ghwas

Background and Objectives: Bacterial cellulose (BC) is a microbial extracellular biopolymer formed by microbial strains like Gluconacetobacter xylinus. The objective of this study was to determine the antioxidant and anticoagulant of a microbial nano cellulose-ZnO-Ag (CNCs) composite and its components separately. Materials and Methods: Three components were used for nano cellulose-ZnO-Ag composite synthesis, Ag-nanoparticles, ZnO-nanoparticles and BC. The DPPH method was used to calculate the scavenging of free radical behaviour of four different composite samples. Results: Results of silver nanoparticles were found to have the highest antioxidant activity with IC₅₀ 65 μg mL^–1, followed by CNCs-ZnO-Ag composite (IC₅₀ 88.98 μg mL^–1) but ZnONPs IC₅₀ was 263 μg mL^–1 and BC (IC₅₀ 955 μg mL^–1). The CNCs-ZnO-Ag composite, BC and AgNPs at 25 μg mL^–1 had clotting times that were nearly identical to the control. The APTT increased to 56 Sec at 75 μg mL^–1 of CNCs-ZnO-Ag composite related to control that recorded 33 Sec. Conclusion: Bacterial cellulose acquired new activity in nano form and also when conjugated with nanoparticles. The CNCs-ZnO-Ag composite is ready for pharmaceutical application as an antioxidant and anticoagulant after in vivo study.

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