CEG-AgNPs Ameliorates DMBA-Induced Mammary Carcinogenicity by Alleviating Cytokines Expression

Ali A. Ali, Mohammed A. Hussein, Ahmed A. Emara, Ahmed M. Abd Elrahman, Abdelrahman A. Hassan, Ahmed A. Abdelghaney, Ahmed M. Bastawey, Ahmed M. Maher, Abdul-Malik N. Al-Wadayi, Mohamed A. Shalaby, Mohamed M. Mohamed, Mohamed A. Gamal El Din, Saleh A. Muhammad, Ahmed S. Ewees, Mohamed S. Nasr-Eldin, Diana A. Alshrief, Ahmed H. Mohamed, Hala Mostafa, Amr A. El-Ella, Mostafa A. Abdel-Maksoud, Ali A. Ali and Mohammed A. Hussein

Background and Objective: For more than a decade, breast cancer has been one of the most common forms of cancer among women around the world. The present article aimed to evaluate the protective activity of CEG-AgNPs against DMBA-induced mammary carcinoma. Materials and Methods: In this experimental study, green synthesis and characterization of CEG-AgNPs were carried as well as IC₅₀ against Mcf7 cell line and LD₅₀ on mice were evaluated. A total of 24 adult albino mice were divided into four groups six rats in each. Group I was given an equal amount of distilled water, group II was received 80 mg kg^–1 b.wt., DMBA for 4 weeks, groups III and IV were treated with CEG-AgNPs (28.1 and 70.25 mg kg^–1) from the 5th week of DMBA administration for 4 weeks, respectively. Results: CEG-AgNPs were approximately 42.32±9.52 nm with a negative zeta potential of -17.44. It is IC₅₀ against the Mcf7 cell line and LD₅₀ is equal to 82.76 μg mL^–1 and 1405 mg kg^–1 b.wt., A significant normalization in plasma ALT, AST, AST and LDH as well as mammary MDA, TNF-α, IL-6, P53, SOD, GPx and GSH levels have been observed in CEG-AgNPs treated mice. Oral CEG-AgNPs administration has suppressed VEGF-C gene expression in DMBA-treated mice. Conclusion: The present results, biochemical, histological and MRI results showed that CEG-AgNPs have potent anticancer activity against DMBA-induced mammary carcinoma in mice by inducing the biosynthesizes of antioxidant biomarkers and suppression of cytokines gene expression.

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