Root Inoculation with Pseudomonas putida IFO 14796 for Improving Iron Contents in Maize Grain

Jumadi , Saifuddin Sirajuddin, M. Natsir Djide and Anwar Mallongi

Background and Objective: Anemia that caused by intake the low iron staple food is a global issue. Biofortification through PGP (plant growth promotion) intervention was a new strategy to improve mineral content in staple food. This study aimed to improve iron content in maize grain through root inoculating with Pseudomonas putida IFO 14796. Materials and Methods: Experiment was carried out by randomized group design. Roots plant was inoculated P. putida IFO 14796 (5.18×10⁷ C.F.U g^–1) used as treatment and uninoculated as a control. The stem size mean of corn plant was measured after 55 days in cultivated. The iron contents in maize grain after 30 DAP (days after pollination) were analyzed using the Atomic Absorbance Spectrophotometer (AAS) method. Results: The stem size mean of the corn plant with the treatment of P. putida IFO 14796 was 65.09 cm and control 52.39. The stem size mean of corn plant was significantly different at p<0.05. The higher iron content in maize grain 10.1117 mg kg^–1 was obtained from root inoculated P. putida IFO 14796, while uninoculated was 8.5130 mg kg^–1. Improving iron content in maize grain up to 18.79% after 30 DAP (days after pollination). Conclusion: It is concluded that Iron content in maize grain can be improved through root inoculating with P. putida IFO 14796.

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