Profilin and Vicilin Genes of Allergens in Fingerprinting of Minor Legumes

Adam Kováčik, Jana Žiarovská, Aqsa Abbas and Dagmar Moravčíková

Background and Objective: Coding regions of plants are a good source for designing the DNA markers to describe genetic variability in specific genomic parts. Here, the genetic variability of five selected minor legume species was analyzed by DNA markers for profilins and vicilins. Materials and Methods: Varieties of Phaseolus vulgaris L., Cicer arietinum L., Pisum sativum L., Lens culinaris Med. and Vicia faba L. were analyzed by profilin-based amplified polymorphism (PBAP) and vicilin based amplified polymorphism (VBAP) methods. The heterozygosity index, polymorphism information content, effective multiplex ratio, marker index, diversity index and discrimination power were calculated by iMEC. Dendrograms were constructed based on the obtained binary matrices using the Jaccard coefficient of genetic similarity. Polymorphism and marker techniques effectiveness were compared. Results: Profilin-based polymorphism was found to be more effective in generating polymorphic fingerprints in all the analyzed legume species. Vicilin-based polymorphism provides only a species-limited tool for genomic variability analysis in common beans and chickpeas. Conclusion: Profilin-based polymorphism is more effective in generating polymorphic fingerprints. Vicilin-based polymorphism provides a specie-limited tool for genomic variability analysis in legumes.

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