Journal of Medical Sciences

Volume 22 (4), 158-164, 2022

Facebook Twitter Linkedin WhatsApp E-mail
Evaluating Cytochrome P-2E1 Induction in Diabetic Rats and Rabbits

Asieh Karimani

Background and Objective: Diabetes is one of the most complicated health problems worldwide. Alteration in the amount of CYP450 enzymes during this disease may affect the metabolism of drugs and xenobiotics. In this study, the severity of CYP2E1-induction in different weeks after the onset of diabetes is compared in two different but useful species of experimental animals, rats and rabbits. Materials and Methods: Thirty male Wistar rats and 24 adult male New Zealand rabbits were randomly divided into two groups, diabetics and non-diabetics. Streptozocin (50 mg kg1, i.p.) and alloxan (100 mg kg1, i.v.) were injected as diabetogenic agents respectively in rats and rabbits. In 3 subsequent weeks (3, 4 and 5 weeks post diabetes induction in rats and 5, 6 and 7 weeks post diabetes induction in rabbits) animals were sacrificed and CYP2E1 proteins levels were determined by western blotting. Results: The volume of CYP2E1 protein in diabetic rabbits was significantly higher at 6 weeks post diabetes induction than 5 and 7 weeks post diabetes induction (p<0.01, p<0.05, respectively). Moreover CYP2E1 level was higher in the 7th week compared to the 5th week (p<0.05). The CYP2E1 protein levels in rats who were sacrificed 4 and 5 weeks after diabetes induction were significantly higher than the rats that were killed in the 3rd week (p = 0.05). Moreover, the complications in inducing diabetes in these two experimental animals are elaborated. Conclusion: The present results showed that diabetes could induce the amount of CYP2E1 proteins in rats and rabbits by the optimum value in the 4th and 6th week after diabetes induction.

View Fulltext Back

How to cite this article:

Asieh Karimani, 2022. Evaluating Cytochrome P-2E1 Induction in Diabetic Rats and Rabbits. Journal of Medical Sciences, 22: 158-164.

DOI: 10.3923/jms.2022.158.164

Article Statistics