Effectiveness of Superdosing Phytase to Low Phosphorus and Calcium Diet on Growth Performance, Nutrient Digestibility, Blood Parameters of Broiler Chickens

Md Rana, Md Sazedul Sarker, Md Islam, Md Hassan and Md Aftabuzzaman

Objective: This study was designed to investigate the Effects of superdosing phytase to low available phosphorus (avP) and calcium (Ca) diet on growth performance, serum biochemical, digestibility and phosphorus excretion in broiler chickens. Materials and Methods: A total of 375 one-day-old broiler chicks were randomly assigned to 5 treatments with 5 replicates. Treatments consisted of PC, positive control (adequate avP and Ca); NC, negative control (low avP and Ca) and the NC group supplemented with 3 levels of phytase (5000, 10000 and 15000 FTU/kg diet). Body weight and feed intake of birds were recorded at 11, 25 and 42 days of age. Blood samples of ten birds from each treatment were collected from wing venous at 21 and 42 days of age to evaluate serum biochemical indices and inositol in plasma. A digestibility trial was conducted to assess the apparent dry matter, crude protein, metabolizable energy and content of P excretion in feces at the end of the experimental period. Results: In all rearing period, phytase-treated NC diet group of birds showed improved body weight (p<0.01) and weight gain (p<0.01) similar to PC diet. However, birds fed high phytase doses gained more body weight and weight gain. The highest dose (15000 FTU/kg) increased serum albumin (p<0.05) at 42 days and serum IL-2 and IL-6 at a similar level, which was statistically equivalent to PC diet. On 42 days, serum protein and plasma inositol levels increased in response to all doses of phytase. On 42 days, serum Ca and P values differed by treatment, with NC diet having the lowest value. Birds fed NC or phytase treated diets had similar levels of P excreta. Conclusion: The super dose of phytase supplementation to a low avP and Ca diet can improve broiler performance and serum protein, calcium and phosphorus levels in the blood.

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