Effects of Ferulic Acid Regulation of the PI3k/Akt Signaling Pathway on the Proliferation, Migration or Apoptosis of U87-MG Cells Based on Bioinformatics

Peng Jin, Zhenfei Zhao, Xue Li, Qingbu Mei, Dan Liu, Muqi Liu and Kun Zhao

Background and Objective: Glioma is a common brain tumour. Most deadly brain tumours are malignant. Traditional glioma treatment includes surgical resection, radiation, chemotherapy and biological therapy. Through a series of in vitro research studies, this study was intended to assess the effects of ferulic acid (FA) on the proliferation and tumour features of U87-MG cells. Materials and Methods: The U87-MG cells were employed in this study. Blank and ferulic acid groups with concentrations of 1.25, 2.50 and 5.00 mmol/L were created. The FA’s effect on U87-MG cell apoptosis was examined by AM/PI staining after cell prolifer activity. The ELISA, cell migration, transwell invasion test, intracellular reactive oxygen species, cell apoptosis rate and western blot were assessed. Results: The application of FA decreased the proliferation of U87-MG cells, according to the results of the cell counting kit 8 test. Flow cytometry analysis also showed that FA sped up the death of cells and greatly increased the amount of reactive oxygen species inside cells. The phenotype of FA promoting apoptosis in U87-MG cells was consistent with the results of western blot analysis, which demonstrated that ferulic treatment with acid elevated the expression of apoptotic proteins (caspase-3 and caspase-9) and decreased the expression of cell cycle-related proteins (cyclin) in U87-MG cells. The FA suppressed U87-MG cell growth by blocking the PI3K/Akt signalling pathway, according to further detection of this pathway’s activity. Conclusion: The FA treatment boosted autophagy in U87-MG cells by increasing autophagy protein expression. The clinical treatment of gliomas changed with this discovery.

View Fulltext Back