Antitumoral Effect of Tomentosin from Inula viscosa Plant on Mice with Ehrlich Acid Tumor

Izzettin Guler and Mehmet Ozaslan

Background and Objective: Spontaneous or transplantable tumor models are kinetically similar to human cancer types and play a role in understanding the biology of carcinogenesis and in the development of chemo-preventive and chemo-suppressive drugs. In this study, the effect of tomentosin isolated from the Inula viscosa plant against Ehrlich ascites tumor (EAT), was carried out. Materials and Methods: Inula viscosa (Asteraceae) was collected from the vicinity of Kilis 7 Aralık University, Kilis, Turkiye and tomentosin was isolated. In this study, 7 groups of 6 mice were used in each group. The EAT was taken from stock mice and counted in a Cedex XS cell counter (Roche) and administered intraperitoneally to tumor groups in 0.2 mL PBS (phosphate-buffered saline) once at the beginning of the study. Tomentosin was administered intraperitoneally to groups in 3 doses (25, 50 and 100 mg/kg). Average weight change, total oxidant capacity (TOS), total antioxidant capacity (TAS) and superoxide dismutase enzyme (SOD) activity were examined. The liver, small intestine, large intestine and stomach were taken from sacrificed animals and examined histopathologically. The findings were statistically evaluated using ANOVA followed by Dunnett’s Multiple Comparisons test in SPSS 17, with significance set at p<0.05. Results: The weight changes, SOD and TAS results of mice showed that tomentosin at a dose of 100 mg/kg reduced the tumor. It was determined that tumors in groups where tomentosin was administered together with tumors were morphologically reduced and this reduction was directly proportional to the dose of tomentosin. In the group in which 100 mg/kg tomentosin dose and Ehrlich ascites tumor were administered together, histopathological examination of kidney, small intestine, large intestine and stomach tissues showed less tumor involvement compared to other doses. Conclusion: Weight, biochemical and histological evaluations supported each other that tomentosin is effective in reducing tumors and it is thought that this study will be useful for future studies.

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