Analysis of Coffea canephora Bean Supercritical Extract and Raffinate and Development of Active Material

Shinsung Park, So Park and Su Park

Background and Objective: Coffea canephora bean contains a large amount of caffeine and chlorogenic acid, which have effects such as anti-cellulite and hair-loss improvement. This study aimed to find an optimal extraction process that maximizes active ingredients from Coffea canephora beans and to develop a functional material improved transdermal permeability. Materials and Methods: The nine extracts were quantitatively and qualitatively analyzed on caffeine and chlorogenic acid with HPLC. Polyphenol quantification assay, DPPH radical scavenging assay and ABTS+radical scavenging assay were performed. A deformable liposome encapsulating the optimum Coffea canephora bean extracts was prepared using propylene glycol to improve stability and transdermal permeability. The characteristics of formulations were measured and Franz diffusion cell was performed to confirm the transdermal permeability. Results: Based on HPLC analyses and radical scavenging assays, the supercritical extract under the condition of 150 bar and 60°C and the hydrothermal extract of the supercritical raffinate were selected as the optimum extracts. The deformable liposome system dramatically enhanced the stability and skin permeation of the formulation. The transdermal permeability was increased 15.35 fold. Conclusion: The analyses verified that the best extraction method for caffeine is the supercritical extraction at 150 bar and 60°C and that the best extraction method for chlorogenic acid is the hydrothermal extraction of supercritical raffinate. In addition, the results suggest that the formulation with the optimum Coffea canephora bean extracts could be used as an excellent functional material with abundant active ingredients, higher stability and improved transdermal permeability.

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