Background and Objective: Post-anesthesia inflammation in retinal cells is a remarkable ocular complication that could lead to vision issues. This study was aimed to investigate the mechanism of action of Ganoderma lucidum polysaccharides (GIP), a naturally derived extract, on the inflammatory response of retinal cells. Materials and Methods: The GIPs were extracted and the total sugar content was determined by the phenol-sulfuric acid method, with reducing sugar content assessed using the DNS method. In vivo experiments, 50 Sprague-Dawley rats were selected to establish a model of post-anesthesia retinal cell inflammation and were divided into control, model and three GIP treatment groups (treated with 50, 100 and 150 mg/kg of GIP, respectively). Comparative analyses included retinal electrophysiological examinations, retinal thickness measurements and evaluation of inflammatory factors. In vitro experiments, retinal cells were cultured to analyze the expression level (EL) of Bcl-2, Bax and Caspase-3 in various groups. The PCR analysis was performed to determine the relative mRNA ELs of NF-κB, LOX-1, TRAF-6 and TLR-4 in the various groups. Results: The cold-water immersion method yielded consistent GIP extraction rates around 39%, with negligible differences (p>0.05). In vivo, GIP treatment significantly increased a-wave and b-wave amplitudes in retinal electrophysiology compared to the model group, showing dose dependency (p<0.05). The GIP administration also decreased inflammatory factors (VEGF, TNF-α, IL-1β, IL-6, ICAM-1 and IL-18) in rat serum and inhibited retinal cell apoptosis. In vitro, GIP treatment reduced mRNA expression levels of NF-κB, TLR-4 and TRAF-6, indicating its potential to mitigate inflammation and promote cell survival (p<0.05). Conclusion: The cold-water immersion methodology is effective in extracting GIPs with repeatability and reliability. The natural extract of GIPs dramatically inhibits the inflammation of post-anesthesia retinal cells, potentially involving the modulation of the NF-κB signaling.
Na Li, 2024. Mechanism of Ganoderma lucidum Polysaccharides Mediating NF-κB Signaling Pathway-Related Proteins Alleviating Post- Anesthesia Inflammation in Retinal Cells. International Journal of Pharmacology, 20: 1488-1500.