Resveratrol Extract on the Protein Expression of Inflammatory Factors in Osteoarthritis Chondrocytes

Hougen Lu and Jian Xiong

Background and Objective: Osteoarthritis is mainly the degenerative destruction of articular cartilage and joint inflammatory response. This research aimed to discuss the outcome of resveratrol (RES) extract on the protein expression of inflammatory factors in osteoarthritis chondrocytes. Materials and Methods: Human osteoarthritis cartilage cell line (HC-OA) was used as the research material and HC-OA were induced by Interleukin-1β (IL-1β) to simulate the joint inflammatory environment. The cell viability was tested by Cell Counting Kit-8 (CCK-8) method. The expression of type collagen in the cells was tested by immunocytochemical staining of type collagen. The cell proliferation cycle was tested by flow cytometry (FC). The protein and mRNA levels of Toll-Like Receptor 4 (TLR4) and Nuclear Factor-κB (NF-κB) at were tested by western blot (WB) and Real-Time Fluorescent Quantitative PCR (RT-PCR). Tumor Necrosis Factor-α (TNF-α) and Interleukin-6 (IL-6) were tested by Enzyme-Linked Immunosorbent Assay (ELISA) to evaluate the improvement effect of RES on the protein expression of inflammatory factors. Results: As against the IL-1β, the activity of HC-OA in the IL-1β+RES was obviously enhanced and the proportion of HC-OA in G0/G1 phase was obviously raised. The TLR4 mRNA, NF-κB mRNA, TLR4 and NF-κB protein in HC-OA of IL-1β+RES had an obvious decrease. The protein expression of IL-6 and TNF-α in chondrocytes of IL-1β+RES group was obviously decreased and the protein expression of IL-10 had a clear increase. Conclusion: The RES could clearly improve the protein expression of inflammatory factors in osteoarthritis chondrocytes, indicating that RES can be adopted as a possible therapy drug for osteoarthritis and provide a new idea for clinical treatment.

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