Effects of Picroside Regulating the lncRNA-MALAT1/miR-21/PPAR Signaling Pathway in Fat Synthesis of Patients with Gestational Diabetes Mellitus

Dan Liu, Yang Zhang, Guxiang Zhang and Chengfang Xie

Background and Objective: The regulatory mechanism of picroside on fat synthesis in Gestational Diabetes Mellitus (GDM) remains unclear at present. This work investigated the regulatory effect of picroside on fat synthesis in patients with GDM and analyzed the roles of picroside in regulating the lncRNA-MALAT1, miR-21 and PPAR-γ signaling pathways (SPWs) in its effects on fat synthesis. Materials and Methods: Picroside was extracted and purified. Human subcutaneous adipocytes (3T3-L1) were divided into control (Ctrl), model (Mod) and picroside groups. Triglyceride (TG) and total cholesterol (TC) were detected and lncRNA-MALAT1 and miR-21 were measured. Protein levels of Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ), CCAAT/Enhancer-Binding Protein Alpha (C/EBP-α), etc., were determined. Results: The highest concentration of picroside extracted after 3 hrs using 95% ethanol was 279.25 mg/g. After purification with D201 macroporous adsorption resin, the mass fraction of picroside was 43.23%. In the Mod group, levels of TC, TG and lncRNA-MALAT1 were markedly elevated versus the Ctrl group (p<0.05). In picroside group, TC, TG and lncRNA-MALAT1 levels were superior to the Mod group (p<0.05). Conclusion: These findings indicated that picroside regulated fat synthesis in patients with GDM through the lncRNA-MALAT1/miR-21/PPAR-γ SPW.

View Fulltext Back