Identification of Clinical Pseudomonas Pf Phages Inducible by Mitomycin and Investigation of the Antibiotic Relevance of Pf1 Phage

Nawal Al Aazi, Yasemin Zer, Zeynep Çelik, Uğur Vural, Enes Boyraz, İbrahim Kilic and Mehmet Özaslan

Background and Objective: Pseudomonas aeruginosa boasts a significantly expansive genome relative to its microbial counterparts. This genome size endows Pseudomonas aeruginosa with the capacity to thrive across diverse environments, generate an array of virulence factors and manifest resistance against a wide spectrum of antibiotics. Notably, Pseudomonas aeruginosa is responsible for both community-acquired and hospital-acquired infections. In this study, Pseudomonas aeruginosa isolates were subjected to mitomycin C treatment and bacterial isolates with all induced filamentous Pf’s, including Pfu-a, Pfu-b, Pf1, Pf4 and Pf5, were selected and it was investigated how the presence of Pf1 differed in these isolates. Materials and Methods: The study included 125 clinical isolates of Pseudomonas aeruginosa that were identified using automated systems from patient samples that were requested from different clinics of Gaziantep University Şahinbey Research and Application Hospital. The ethical permission for the collection of Pseudomonas aeruginosa bacterial isolates was acquired on September 18, 2019, with decision number 2019/275 from the Gaziantep University Clinical Research Ethics Committee. Antibiotic susceptibilities were also determined for each isolate. For each sample, ethical guidelines were followed to record and proceed analysis. Results: The presence of Pfu-a, Pfu-b, pf4 and Pf5 filamentous phages were detected by PCR. The presence of Pf1 filamentous phage was investigated in 33 clinical Pseudomonas aeruginosa strains that were detected as filamentous phage positive. The correlation between the presence of Pf1 filamentous phage and antibiotic sensitivities was investigated. Conclusion: The presence of pf1 phages in Pseudomonas aeruginosa genome is associated with antibiotic effects.

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