Biological Evaluation of Chinese Herbal Compounds as Anticancer Agents in Fighting Against Lung Cancer

Xi Zhao and Salam Pradeep Singh

Background and Objective: The survival rate for lung cancer has not improved in recent years despite various treatments and therapy. Therefore, the present study attempt to investigate compounds of natural origin and natural source as a new lead for anti-lung cancer therapy. Materials and Methods: A549 cancer cell line assay was carried out against seven Traditional Chinese Herbal Compounds. It was followed by an interleukin assay against IL-10, IL-1β and IL-17A. The compounds were further checked for their molecular interaction at the active site of these enzymes using molecular docking simulation. Results: Scutellarin, glycyrrhizic acid, ginsenoside and astragaloside could inhibit the growth of A549 cells growth from 68-89%. These herbal compounds also inhibited IL-10, IL-1β and IL-17A with an IC value ranging from 8.41±0.014 (scutellarin in IL-1β) to 17.44±0.019 μM (astragaloside in IL-17A). The result of the molecular docking simulation observed that these four herbal compounds docked at the active site of IL-10, IL-1β and IL-17A with favourable docking scores. Conclusion: Scutellarin, glycyrrhizic acid, ginsenoside and astragaloside reduced the growth of A549 lung cancer cells. The ELISA assay also confirms the inhibition of IL-10, IL-1β and IL-17A which is consistent with the molecular docking result.

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