Ovothiol-A Ameliorates Renal Injury Induced by Bile Duct Ligation in Rats (Biological, Quantum-Chemical and Molecular Docking Study)

Nada Mahmoud Khalil Madany, Mohamed Refaat Shehata, Ayman Saber Mohamed and May Mohamed Elbatran

Background and Objective: Cholemic nephropathy is determined as Cholestasis-induced kidney damage. Ovothiols extracted from marine animals especially sea urchins display awesome antioxidant characteristics due to the thiol group’s peculiar position on the imidazole part of histidine. This study investigated the protective effect of Ovothiol-A contra kidney dysfunction induced by bile duct ligation in rats. The chemical properties of Ovothiol-A are estimated by Quantum chemical approaches (DFT B3LYP/6-311G++), Frontier orbitals, MEP and Molecular docking. Materials and Methods: Thirty rats (male) were randomly allocated into three groups: BDL, Sham and BDL + 500 mg kg^–1 Ovothiol-A. All rats were treated for 7 days. (Sham group and BDL group were administered with distilled water, another group was administered with Ovothiol-A). Oxidative stress biomarkers and kidney function tests were then evaluated in all groups of rats. Histopathological examination of the kidney was also evaluated. Results: Ovothiol-A decreased concentrations of urea, creatinine, uric acid, MDA, NO and increased the levels of CAT, GSH, SOD and GST. Microscopic examination of the kidneys of Ovothiol-A-treated rats revealed normal structure without noticeable pathological alterations. Conclusion: Ovothiol-A is a potent antioxidant substance that protects the kidney against toxicity results from BDL in rats.

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