Effect of Roasted Date Palm Rich Oil Extracts in Liver Protection and Antioxidant Restoration in CCl₄-induced Hepato Toxicity in Rats

Hussein M. Eldeeb, Hamdoon A. Mohammed, Mohammed S. M. Sajid, Salah Elnaeem M. Eltom, Mohsen S. Al-Omar, Mugahid A. Mobark and Amira S. Ahmed

Background and Objectives: Roasted date palm seeds are commercially available in the markets and used as hot-coffee drink-substitute and traditionally in the treatment of male infertility. This study aimed to evaluate the lipid profile, hepatoprotective, in vivo antioxidant activities of roasted date palm rich oil extracts on carbon tetrachloride (CCl₄) induced oxidative liver injury in rats. Materials and Methods: Rats were divided into 5 groups (n = 5), placebo (G1), rats received CCl₄ (G2), rats received CCl₄ and silymarin (G3), rats received CCl₄ and roasted date palm seed hexane extract (RDPHE) (G4) and rats received CCl₄ and roasted date palm seeds chloroform extract (RDPCE) (G5). Liver functions and lipid profile were determined by spectrophotometric assays. The superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were assayed by ELISA. Results: Both extracts of roasted seeds significantly decreased ALT level compared to G2 and significantly improved total cholesterol compared to both G2 and G3. The activity of SOD and GPx were significantly enhanced in G3, G4 and G5 compared to G2. Significant negative correlations of ALT with total cholesterol and HDL-C and also between ALP and LDL-C were recorded. Clear preservation of tissue architecture, intact hepatocytes, disappearance of tissue necrosis, fibrosis and marked reduction in inflammatory cellular infiltration were observed in the liver tissues of the treated groups. Conclusion: In conclusion, RDPHE and RDPCE enhanced the antioxidant enzymes activity and have ameliorative effects against CCl₄-induced hepatic injury in rats. The significant negative correlation was observed between liver enzymes (ALT, ALP) and lipid profile.

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