Development of Diabetic Animal Model via Insulin Glargine Induction

Tyas Rini Saraswati, I. Gusti Ayu Agung Suartini and Silvana Tana

Background and Objective: Diabetic patients depend on insulin, however a long-term insulin treatment caused insulin resistance which is caused by Insulin Receptor substrate (IRs) damage. This study aimed to get animal diabetic model caused by insulin resistance induced by insulin glargine. Materials and Methods: The experiment was performed with fully random design. Male Rattus novergicus Wistar is induced by glargine insulin 1.80 IU kg^–1 weight per day, every day for 14 and 28 days and compared with control mice. Injection used as subcutaneous on the abdomen by using xiulin pen. At the end of the treatment, the samples have fasted for 10 hrs. The blood was taken directly from the heart after dissection. The glucose level was compared between treatments. Side effect parameters were observed to the consumption of food, drink, lipid profile, body weight, liver function and liver histology description. Results: The results showed that glargine insulin induction at the dosage of 1.80 IU kg^–1 weight per day for 28 days caused hyperglycemic with 117,33 mg dL^–1 fasting glucose level in blood followed by the increase of food and drink consumption, liver weight and the change in lipid profile. Conclusion: Glargine insulin induction at 1.80 IU kg^–1 weight per day for 28 days caused pre-diabetic in male Rattus novergicus.

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