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Current Research in Bacteriology
eISSN: 2077-2122
pISSN: 1994-5426

Editor-in-Chief:  Gabriel Olaniran Adegoke
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Research Article
Published on December 15, 2016
Species Identification of Clinical Coagulase-negative Staphylococci Isolated in Al-Shifa Hospital Gaza using Matrix-assisted Laser Desorption/Ionization-time of Flight Mass Spectrometry
Nahed Ali Al Laham
Background: Staphylococcal species, notably, coagulase negative staphylococci (CoNS) are considered important causative agents of hospital-acquired infections (HAI) associated with catheters and implanted medical devices. The increasing incidence of CoNS in HAI confirms the need for an accurate and simple identification method at the species level. Here, the capacity of MALDI-TOF MS was compare in comparison of VITEK-2 and ID32STAPH to accurately identify clinical CoNS species and to determine its ability to correctly distinguish between methicillin resistant CoNS (MR-CoNS) and methicillin sensitive CoNS (MS-CoNS). Materials and Methods: In this study, MALDI-TOF MS, VITEK-2 and ID32STAPH was compared for phenotypic identification of CoNS at species level. Eighty one clinical isolates of CoNS representing six species that collected from different clinical samples were analyzed. All CoNS isolates were tested by catalase, Pastorex™, coagulase, nuc and mecA genes. Statistics were performed with Statistical Package for Social Sciences (SPSS®) program version 20 (Chicago, IL, USA). Results: Our results showed correct species identification by MALDI-TOF MS was obtained in 98.8% in comparison to 96.3 and 75.3% correct identification for the VITEK-2 and ID32STAPH, respectively. Only one strain was identified by MALDI-TOF MS at the genus level. In addition, MALDI-TOF MS identified staphylococcal to subspecies level including Staphylococcus hominis subsp., novobiosepticus and subsp., hominis, S. saprophyticus subsp., saprophyticus. Also the single strain of S. auricularis was only identified by MALDI-TOF MS. There were 60 (74.1%) MR-CoNS that showed mecA positive and 21 (25.9%) were MS-CoNS. The MALDI-TOF MS technique was unable to discriminate between MR-CoNS and MS-CoNS, because the topology of dendrogram generated from the spectra of MR-CoNS and MS-CoNS strains were almost the same. Conclusion: These results confirm the value of MALDI-TOF MS as simple, accurate and rapid method for phenotypic identification of clinical CoNS isolates. However, this technique was unable to discriminate between MR-CoNS and MS-CoNS strains.
Research Article
Published on December 15, 2016
Occurrence of Antibiotic Resistance and Virulent Factors in Enterococcus faecalis Isolated from Bush Meat Roasted and Sold along Road Sides in Ekiti State
Olawale Adetunji Kola, David Oluwole Moses, Onasanya Amos, Ajayi Ayodele Oluwaseun, Osuntoyinbo Richard Tope, Idris Olayinka Oluwatoyin and Oje Opeyemi James
Background and Objectives: In Nigeria wild animals are hunted for meat mainly. Meanwhile, meat from wild animals are known to consist of pathogens apart from being contaminated by unhygienic environments (road sides) where they are mainly prepared and sold. The objective of this study was to investigate the occurrence of Enterococcus faecalis (E. faecalis) in samples of roasted bush meat prepared and sold along Ado-Ekiti-Ilesha road. Materials and Methods: A total of 182 roasted bush meat samples were collected in seven selected towns in Ekiti State, Nigeria between January and February, May and June, 2016 representing dry and rainy seasons, respectively. The samples were examined for the presence of enterococci within 2 h of collection. Standard methods were used to identify Enterococcus faecalis, determined its resistance to antibiotics and also determine the virulence factors in the sample. Results: A total of 91 (32.38%) out of 281 samples of roasted bush meat examined were contaminated with E. faecalis. The highest rate of contamination 61.22% was observed in samples collected from Igede-Ekiti while the least 6.70% was observed from samples collected in Ado-Ekiti. Rates of contamination among samples from other selected towns were, 40, 38.71 and 38.1% from Efon-Alaye-Ekiti, Iyin-Ekiti and Erio-Ekiti, respectively. Antibiotic susceptibility test results reveal that some of the isolates have acquired resistance to a number of antibiotics. High resistance rate was recorded against ampicillin 35.71%, followed by gentamicin 30.22%, ciprofloxacin 28.02% and ofloxacin 24.73%. The incidence of virulence factors was low in all the isolates with aggregation substance, haemolysin and gelatinase recording 7.69, 8.24 and 27.47%, respectively. Conclusion: The incidence of virulence factors in E. faecalis is an evidence of potential pathogenesis. The roasted bush meat screened from road sides in Ekiti State was contaminated with E. faecalis. There is need for strict monitoring and proper hygiene education for the food handlers in the study area.

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