Investigation of Some Histological, Physiological and Microbiological Properties of Honey Types and Vaseline as Medications of Second Degree Burns in Rats

Roqayah Kadi, Rokayya Sami, Manal Almatrafi, Suzan Harara, Rasha Al-Eisa, Ruqaiah Bedaiwi, Helal Al-Harthi, N.I. Aljuraide, Fadi Baakdah, Zeyad Alharbi, Mohammad Alanazi and Fahad Albalawi

Background and Objective: Honey is a natural product with high osmolality and acidity, it affects the cytokines production by skin cells when applied as a coating treatment. The current study aimed to investigate some histological, physiological and microbiological properties of honey types and vaseline as medications for burns in rats. The irradiated honey types are named Nigella sativa honey (NSH), moringa honey (MOH), sidr honey (SIH) and pumpkin honey (PUH), respectively. Materials and Methods: The 60 adult male albino rats were divided equally into 6 sets. Second-degree burns were induced, while animals in (3, 4, 5 and 6) groups were treated with irradiated honey (NSH, MOH, SIH and PUH) as ointment coatings daily, while group 1 was treated with vaseline, respectively. Clinical assessments included histopathological analysis such as the fibroblasts and vessel density ratios. The wound diameter and color of the dermis were evaluated. The microbial infection was detected against Escherichia coli on burned wounds. Each wound was evaluated on days 0, 5, 10 and 15 until the full healing. Results: The fibroblast ratio on the 15th day was recorded at 68.44, 72.99, 74.53 and 63.70 for (NSH, MOH, SIH and PUH) compared to the vaseline (77.21). The highest vessel density ratio was detected for the treated rats with MOH (15.87). The wound diameter reached the maximum diameter contraction on treated rats with PUH (2.37 mm). The microbial population against Escherichia coli reached the maximum on treated rats with vaseline group (8.89 Log CFU/mL). Conclusion: The topical use of PUH followed by SIH and NSH enhanced the healing processes of burned rats.

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