Analysis of Ingredients of Biyuan Tongqiao Granule Absorbed into Blood in Rats Based on UPLC-QTOF-MS Technology

Xin Yang, Xue Di, Tian Li, Shuai Wang, Yong Bao, Xian Meng and Yun Zhang

Background and Objective: Biyuan Tongqiao granule is the Chinese patent medicine commonly used in the treatment of acute and chronic rhinitis and sinusitis. It is composed of 14 Chinese medicinal herbs. In this study, serum pharmacochemical methods were employed to analyze the prototype blood components of Biyuan Tongqiao granule after oral administration in rats, aiming to provide potential quality markers for the quality control of Biyuan Tongqiao granule. Materials and Methods: The UPLC-QTOF-MS technique was used in combination with PCDL database to analyze and investigate the difference in the chromatograms between blank plasma and administered plasma under the same detection conditions. According to mass spectrometry data including retention time, precise molecular mass and secondary fragment ion, the prototype components in the blood of rats were analyzed and identified after administration of Biyuan Tongqiao granule. Results: Totally 25 prototype monomers were detected, which included 11 flavonoids (like narirutin and ononin), 4 organic acids (such as protocatechuic acid and chlorogenic acid), 4 terpenoids (like glycyrrhizic acid and oleanolic acid), 2 quinones (cryptotanshinone and tanshinone IIA), 2 coumarins (scopoletin and isoimperatorin), ephedrine and 2 pseudoephedrine alkaloids. The 25 prototype components in the blood of rats after oral administration of Biyuan Tongqiao granule are derived from 13 out of 14 medicinal herbs in the compound, which can comprehensively control the quality of the compound. Conclusion: Results in this study lay an experimental foundation for the development of quality control methods for the compound.

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