Pakistan Journal of Biological Sciences

Volume 25 (12), 1047-1057, 2022


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Growth Phase Influence the Gene Expression and Metabolite Production Related to Indole-3-Acetic Acid (IAA) Biosynthesis by Serratia plymuthica UBCF_13

Liza Yusfi, Djong Tjong, Irawati Chaniago, Aisyah Salsabilla and Jamsari Jamsari

Background and Objective: The optimization of the indole-3-acetic acid (IAA) producing capability of Serratia plymuthica UBCF_13 has been intensively studied. This work tried to reveal the effect of growth phases on IAA production, gene expression and metabolite synthesis related to the IAA biosynthesis pathway. Materials and Methods: The growth curve and IAA production were measured every 3 hrs. The putative IAA biosynthesis pathway was investigated based on the UBCF_13 genome. To identify the possible pathway of IAA biosynthesis in UBCF_13, we applied the Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) and High-Performance Liquid Chromatography (HPLC) analysis to measure the transcript levels of each gene and indole metabolite production based on tryptophan treatment at different times of incubation. Results: The optimal IAA production on colorimetric assay was at 9 hrs of incubation (initial stationary phase). The level expression of puuC, DDC, oxdA, amiE, nthA and nthB have been upregulated maximum in 3 hrs of culture time (lag phase), except tyrB and ipdC. The highest transcript level of the genes was found in nitrile hydratase genes (nthA and nthB) and indole-3- acetamide (IAM) has been detected as the only intermediate in the crude extract of UBCF_13 thus the IAM pathway may be used to produce IAA. The maximum IAA production on HPLC analysis was found at 21 hrs of incubation (late stationary phase). Conclusion: This study gives a new insight that the best time to measure gene expression and intermediates related to the IAA biosynthetic pathway in bacteria was found at a specific growth phase.

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How to cite this article:

Liza Yusfi, Djong Tjong, Irawati Chaniago, Aisyah Salsabilla and Jamsari Jamsari, 2022. Growth Phase Influence the Gene Expression and Metabolite Production Related to Indole-3-Acetic Acid (IAA) Biosynthesis by Serratia plymuthica UBCF_13. Pakistan Journal of Biological Sciences, 25: 1047-1057.


DOI: 10.3923/pjbs.2022.1047.1057
URL: https://ansinet.com/abstract.php?doi=pjbs.2022.1047.1057

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