Antioxidant Defense of Apis mellifera L. in Response to Chlorophyllin Derivatives: As a Marker of Ecotoxicological Stress

Samar Mamdouh Nassar, Salwa Ibrahim Sebak, Tarek Al-Tayb and Azza Mohamed Elgendy

Background and Objective: Chlorophyllin derivatives, mammal-safe pesticides, are using recently to control many agricultural and medical pests such as lepidopteran and mosquitoes population. The objective of this work was to assess the possible environmental risks for the chlorophyllin derivatives field application, particularly related to pollinating insect, Apis mellifera. Materials and Methods: In this study, the foraging worker honey bees fed on tri-sodium copper and magnesium chlorophyllin LC₅₀ (10^–5 and 3×10^–3 M L^–1, respectively) were used to measure the total antioxidant capacity, superoxide dismutase and glutathione S-transferase. Also, midgut cells were papered for the comet assay. Results: The tri-sodium Cu-chlorophyllin increased the TAC, SOD and GST activities in the body homogenate of honey bees in response to the oxidative stress of both chlorophyll and Cu elements. Conclusion: Furthermore, the results of the genotoxicity experiment revealed that Cu-chlorophyllin caused DNA damage in the gut cells more than Mg-chlorophyllin and its effect might be due to copper elements.

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