Biosynthesis of Copper Oxide Nanoparticles Formation from Pongamia pinnata (L.) Pierre Leaf Metabolites and Their Antibacterial Activity

Parsotam Tramta, Kalpna Rakholiya and Mital Kaneria

Background and Objective: Bacterial resistance is one of the biggest challenges in the world. In this point of view, the present study was designed to synthesize copper nanoparticles using leaf aqueous extract of Pongamia pinnata in search of a new source to overcome the issue of drug resistance. Materials and Methods: Copper Oxide Nanoparticles (CuO NPs) were synthesized using Pongamia pinnata leaf extract. The plant extract was prepared using the decoction extraction method. The characterization was done by various spectral analyses namely UV-Vis spectroscopy, FTIR analysis, SEM and Zeta potential analysis. The antibacterial effect was evaluated by the agar disc diffusion method. Results: The synthesis of CuO NPs was confirmed by its characteristic peak at 339 nm by UV-Vis spectra, while SEM revealed the size in the range of 40.3-101.2 nm and the average size was 58.1 nm. The FTIR confirmed functional groups like alcohol, amines, alkyne, nitrile and halogen in P. pinnata leaf extract. The biosynthesized ZuO NPs showed potent antibacterial activity against four microorganisms, viz Staphylococcus aureus ATCC25923, Listeria monocytogenes ATCC19112, Salmonella typhimurium ATCC23564 and Escherichia coli NCIM2931. Conclusion: The green synthesized P. pinnata leaf-mediated CuO NPs can be further explored as a potential candidate for the antibacterial agent.

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